M2825-15F

MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)

Size

£550.00

SKU:: M2825-15F
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.
Clone:: UIC2
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: CD243, also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), is a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds.CD243 is expressed at low levels in the cell membrane of peripheral blood leukocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumor suppression and is therefore of special interest to oncologists. M2825-15F is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs. Can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:200, Use 10ul to label 1x10e6 cells in 100ul. Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): Requires amtigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate pH 6.0 is recommended. Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Human kidney Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice