M2825-13

MDR Protein (MRP1, Multidrug resistance-associated protein 1, ABCC1, ATP-binding cassette sub-family C member 1, Leukotriene C(4) transporter)

Size

£748.00

SKU:: M2825-13
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1.
Clone:: 3H406 (QCRL-1)
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: MRP1 is a 190kD transmembrane phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines and tumors . Applications: Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.2ug/ml (Anti mouse IgG labeled with HRP). Immunocytochemistry: 1:20-1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations Immunohistochemistry: FFPE sections following antigen retrieval by microwave heating in citrate. Flow Cytometry: 1:20. Cells fixed in PBS, 0.5% paraformaldehyde for 30 minutes, followed by anti-mouse IgG labeled with FITC. (Note: Block and wash with solution containing 0.1% Tween.) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.