M2825-13-ML650

MDR Protein (MRP1, Multidrug resistance-associated protein 1, ABCC1, ATP-binding cassette sub-family C member 1, Leukotriene C(4) transporter) (MaxLight 650)

Size

£1,004.00

SKU:: M2825-13-ML650
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1.
Clone:: 3H406 (QCRL-1)
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Extra Details:: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. MRP1 is a 190kD transmembrane phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines and tumors . Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations Immunohistochemistry: FFPE sections following antigen retrieval by microwave heating in citrate. 1:20. Cells fixed in PBS, 0.5% paraformaldehyde for 30 minutes, followed by anti-mouse IgG labeled with FITC. (Note: Block and wash with solution containing 0.1% Tween.) Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice