M2825-13-HRP
MDR Protein (MRP1, Multidrug resistance-associated protein 1, ABCC1, ATP-binding cassette sub-family C member 1, Leukotriene C(4) transporter) (HRP)
Size
£1,004.00
- SKU:
- M2825-13-HRP
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1.
- Clone:
- 3H406 (QCRL-1)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- MRP1 is a 190kD transmembrane phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines and tumors . Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations Immunohistochemistry: FFPE sections following antigen retrieval by microwave heating in citrate. 1:20. Cells fixed in PBS, 0.5% paraformaldehyde for 30 minutes, followed by anti-mouse IgG labeled with FITC. (Note: Block and wash with solution containing 0.1% Tween.) Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice