M2825-12M

MDR Protein (MRP1, Multidrug resistance-associated protein 1, ABCC1, ATP-binding cassette sub-family C member 1, Leukotriene C(4) transporter)

Size

£787.00

SKU:: M2825-12M
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Non-denatured membranes prepared from the human small cell lung cancer cell line H69AR, which highly overexpresses MRP1.
Clone:: 6D559 (QCRL-3)
Format:: Supplied as a liquid in PBS, 0.7% BSA, 0.09% sodium azide.
Extra Details:: MRP1 is a 190kD transmembrane phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines and tumors. Applications: Suitable for use in Immunocytochemistry, Flow Cytometry, Immunoprecipitation and Transport inhibition. Not recommended for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Immunocytochemistry: use on paraformaldehyde (0.5% in PBS) fixed cytospin preparations Flow Cytometry: 0.2-1ug/ml after fixing cells in 0.5% paraformaldehyde, followed by suitable fluorescent tagged anti-mouse reagent (6) Transport Inhibition: Inhibits the ATP-dependent transport activity of MRP1 in inside-out membrane vessicles. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.