M2750-12A
MCP2 (Macrophage/Monocyte Chemotactic Protein 2, Monocyte Chemotactic Protein 2, MCP-2, C-C Motif Chemokine 8, Chemokine (C-C Motif) Ligand 8, CCL8, HC14, Monocyte Chemoattractant Protein 2, Small Inducible Cytokine A8, SCYA8)
Size
£660.00
- SKU:
- M2750-12A
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to mouse MCP-2, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml
- Extra Details:
- The MCP proteins are members of the CC chemokine family that signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated Tcells, basophils, NK cells, and immature dendritic cells. The MCP family cross-reacts across species. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (sandwich): 0.25-1ug/ml, in conjunction with M2750-08D as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant mouse MCP2. Western Blot: 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant mouse MCP2 is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice