MCM2 (Minichromosome Maintenance Protein 2)

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£863.00

SKU:: M2749-30Z
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Asp143-Ser296 of human MCM2, expressed in E. coli. Uniprot/Swiss Accession: P49736
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: MCM2 (Minichromosome maintenance protein 2; also BM28) is a member of the MCM family of proteins. Although its predicted MW is 102kD, it runs anomalously at 125kD in SDS-PAGE. MCM2 forms a large, 450-600kD prereplication complex with MCM3 through 7 plus HBO1, and associates with the ORC to initiate DNA synthesis. Human MCM2 is 904 amino acids (aa) in length (Accession # P49736). It contains one C4-type zinc finger region (aa329-355) and a P-loop NTPase region (aa485-659) and at least 12 Ser/Thr phosphorylation sites in the N-terminal one-third of the molecule. MCM2 has several potential isoforms. There is an alternate start site at Met10 with or without a 47 aa substitution for aa 109-157, a deletion of aa 31-126, a Gly substitution for aa 113-132 coupled with a 69 aa insertion after Lys476, and a seven aa substitution for aa1-137. Over aa143-296, human MCM2 shares 99% aa identity with mouse MCM2. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detects MCM2 in lysates of Ramos human Burkitt's lymphoma cell line and DA3 mouse myeloma cell line. Immunohistochemistry: 5-15ug/ml detects MCM2 in immersion fixed paraffin-embedded sections of human colon array Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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