M2749-30
MCM2 (Mini Chromosome Maintenance Protein 2, BM28)
Size
£570.00
- SKU:
- M2749-30
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bacterially expressed human Mcm2.
- Clone:
- 3H2645 (CRCT2.1 (D1.9H5))
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- MCM2 (Mini chromosome maintenance protein-2), is a protein involved in the control of DNA replication. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (frozen): 1:500-1:1000, Immunohistochemistry (paraffin): 1:200 Note: Requires antigen retrieval using heat treatment prior to staining of paraffin sections (0.01M citrate buffer, pH 6.0). Optimal working dilutions to be determined by researcher. Recommended Secondary Antibodies: I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Hybridoma: Sp2 myeloma cells with spleen cells from CBA/c F1 mice. Recommended Positive Control Tissue: Tonsil, any tissue with high proliferative index Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice