MCL1 (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1, Bcl-2-like Protein 3, Bcl2-L-3, Bcl-2-related Protein EAT/mcl1, mcl1/EAT, BCL2L3)

Size

£642.00

SKU:: M2749-01N
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-AGVGAGLAYLIR, from human MCL1 at C-terminal. Species sequence homology: Bovine, canine and porcine.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Mcl-1 is an anti-apoptotic member of the Bcl-2 family of proteins. It has a pro-survival role in the early stages of apoptosis, and is thought to prevent cytochrome c release from mitochondria. Mcl-1 is also essential for embryogenesis and for the development and maintenance of B and T lymphocytes. Mcl-1 is a highly regulated protein induced by a wide range of survival signals, including cytokines and growth factors. During apoptosis, Mcl-1 is rapidly degraded by the proteasome pathway, and is also readily cleaved by caspases to produce a potent cell death promoting protein. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2000 Western Blot: 1-3ug/ml, observed in lysates of human lung, in mouse and rat thymus, and in cell line K562 on ~35kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice