M2690-19B

MCAF (MBD1-containing Chromatin Associated Factor, MCAF1, Activating Transcription Factor 7 Interacting Protein 1, ATF7IP, ATF Interacting Protein, ATF-IP, ATFa-associated Modulator, hAM, p621) [KO Validated]

Size

£812.00

SKU:: M2690-19B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa25-100 of human MCAF. Species Sequence Homology: mouse based upon conservation between this ortholog and human MCAF at the site to which the epitope maps.
Format:: Supplied as a liquid in Tris-citrate/phosphate buffer, pH 7.5, 0.09% sodium azide
Extra Details:: MBD1-containing chromatin-associated factor (MCAF) is a 1270-residue mediator in the DNA methylation cycle of a variety of genomic functions. This nuclear protein will bind to the C-terminal transcriptional repression domain of MBD1 to form a blocking complex. This molecule will then prevent transcription from methylated promoters in a histone deacetylation-independent action. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000-1:20,000 Immunohistochemistry (Frozen/paraffin): 1:10-1:500 Optimal dilutions to be determined by the researcher. Positive Control: HeLa Nuclear Extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.