M2690-18
MCP-1/JE (Monocyte Chemoattractant Protein-1)
Size
£377.00
- SKU:
- M2690-18
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- E. coli-expressed mature rat MCP-1
- Format:
- Supplied as a liquid in 0.09% sodium azide, before the addition of glycerol to 40%.
- Extra Details:
- Rat MCP-1/JE (monocyte chemoattracctant protein-1) is a 148-amino acid CC chemokine with a NH2-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con A-stimulated rat spleen cDNA library. This rat MCP-1/JE is 49-amino acid longer than human MCP-1 at 3'-end. This 3'-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and explains for the higher molecular weight (25 kD). In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophils. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice