MBD3 (Methyl CpG Binding Protein 3)

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£837.00

SKU:: M2475-51
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: E. coli-derived recombinant human MBD-3 isoform 2
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: MBD-3 (methyl-CpG-binding domain protein # 3) is a 38-40kD member of the MBD family of transcriptional repressors. Through its MBD, and as part of a large complex of regulatory proteins, it binds methylated DNA, promoting gene silencing during S phase. Although it can homodimerize, it is only active when heterodimerized with MBD-2. Human MBD-3 is 291 amino acids (aa) in length, and contains an N-terminal methyl binding domain (aa's 1-72) and a C-terminal coiled-coil region (aa's 216-245) that may be involved in protein-protein interaction. Human MBD-3 shares 95% aa identity with mouse, rat, and canine MBD-3, with differences essentially limited to the C-terminal 25 amino acids. Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human MBD-3. The detection limit for rhMBD-3 is approximately 5 ng/lane under non-reducing and reducing conditions. Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human MBD-3. The detection limit for rhMBD-3 is approximately 0.5 ng/well. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice