M2429-04

Matrix Metalloproteinase 13 (MMP-13, Collagenase-3)

Size

£869.00

SKU:: M2429-04
Purity:: Purified by Protein G affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human recombinant collagenase-3 protein. Cellular Localization: Cytoplasmic
Clone:: 2Q504 (VIIIA2)
Format:: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:: Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. The MMP-13 gene has been isolated and characterized. This gene is composed of 10 exons and 9 introns and spans over 12.5 kb. The overall organization of the collagenase-3 gene is similar to that of other MMP genes clustered at chromosome 11q22, including fibroblast collagenase (MMP-1), matrilysin (MMP-7), and macrophage metalloelastase (MMP-12), but is more distantly related to genes coding for stromelysin-3 (MMP-11), gelatinase-A (MMP-2), and gelatinase-B (MMP-9), which map outside of this gene cluster. Applications: Suitable for use in Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Frozen and formalin-fixed/paraffin): 5-10ug/ml for 30-60 minutes at RT. Staining of formalin-fixed tissues requires boiling of tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Immunoprecipation: (Native verified) 2ug/mg protein lysate. Use Protein G. Optimal dilutions to be determined by the researcher. Positive Control: Conditioned, serum-free medium from (TPA-treated) human chondrosarcoma SW1353 cells. Placenta, breast, ovarian carcinomas. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice