Matrix Metalloproteinase 14 (MT1-MMP, MMP-14, Collagenase-3)

Size

£831.00

SKU:: M2429-03A
Purity:: Purified by peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide selected from the hinge region of human MT1-MMP (KLH). Species sequence homology: mouse, (95%) and monkey chimpanzee, canine and bovine (100%).
Format:: Supplied as a liquid in Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Matrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes degrade the components of extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. MMPs play an important role in wound healing, apoptosis, bone elongation, embryo development, angiogenesis, cancer metastases, and tissue remodeling within many disease states. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MT1-MMP (MMP-14) is a member of the membrane-type subfamily. Each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of extracellular matrix components and is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix. This protein also activates MMP2 protein, and this activity may be involved in tumor invasion. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunohistochemistry (paraffin): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice