M2425-03
Matrix Metalloproteinase 9 (MMP-9, Gelatinase B, Collagenase 4)
Size
£581.00
- SKU:
- M2425-03
- Purity:
- Purified by protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to residues around 107-117 of human MMP-9.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- Matrix metalloproteinase (MMP) is a family of proteinases that target many extracellular proteins, such as other type of proteinases, growth factors, cell surface receptors and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryo development and wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity is correlated with cancer development (2). One way of MMP regulation is controlling the overall proteinase expression at the transcription step (5). Once synthesized, MMP exists in a latent proenzyme form. The maximum MMP activity requires proteolytic cleavage of MMPs to generate active MMPs by release of the inibitory propeptide domain from the whole molecule (5,6). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice