Matrix Metalloproteinase 9 (MMP-9, Collagenase 4, Gelatinase B)

Size

£429.00

SKU:: M2425-01A
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: E. coli-expressed active rat 92kD type IV collagenase (catalytic domain)
Format:: Supplied as a liquid in 0.09% sodium azide.
Extra Details:: The mammalian matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. After cleavage of a signal peptide domain of about 20 amino acids, the MMPs are secreted in latent forms. Upon activation, the N-terminal propeptide domain is cleaved to generate the active forms of MMP. MMP-9 (92kD type IV collagenase, Gelatinase-B) contain the basic structure of propeptide, catalytic, and hemopexin domains. It is an important proteinase in tissue remodeling. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Immunoprecipitation: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.