M2424-02
Matrix Metalloproteinase 8 (MMP-8, Neutrophil ProCollagenase, Collagenase2)
Size
£793.00
- SKU:
- M2424-02
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- NS0-derived recombinant protein corresponding to Leu21-Pro466 from rat MMP-8.
- Clone:
- 8B34
- Format:
- Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- MMP-8, first described in neutrophils, is a classical type-I collagenase; like MMP-1 it is able to cleave intact type-I collagen at the same site in all three strands. the sequence for MMP-8 is very similar to MMP-1, but until recently it was thought to be produced exclusively by neutrophils. MMP-8 has recently been detected in other cell types. MMP-8 is heavily glycosylated, and the zymogen has a mass of 85kD. The zymogen is quickly activated to the 64kD form, and this breaks down to a cascade of active forms. MMP-8 is not produced by most cell types; it has been found in PMNL neutrophils, arthritic chondrocytes and gingival fibroblasts. MMP-8 levels in quiescent cells and tissues is minimal, and mitogen stimulation or protein concentration is often needed to visualize the bands. In addition, cell types vary greatly in the amount of MMP-8 produced. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot:1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice