M2423-21
Matrix Metalloproteinase 7 (MMP-7, Matrilysin, Matrin, Pump1, Uterine Metalloproteinase, MPSL1)
Size
£800.00
- SKU:
- M2423-21
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Rectal carcinoma (SW837) cells
- Clone:
- 2Q524 (141-7B2)
- Format:
- Supplied as a liquid in PBS, pH 7.0, 2% BSA (protease free)
- Extra Details:
- H-MMP-7 is a member of the Matrix metalloproteinase family of endopeptidases, containing the canonical HExxHxxGxxH motif as a zinc binding active site. MMP-7 and MMP-26 (Matrilysin-2) are the only MMPs discovered that completely lack a hemopexin “regulatory domain” or hinge region. Like the other MMPs, MMP-7 is secreted as a zymogen, then activated. The 28kD form is reduced to 18kD by enzymatic cleavage after the conserved “cysteine switch.” MMP-7 is not constitutively produced. It is produced on demand in specific tissues. MMP-7 substrate specificity is broad, most closely resembling the activity of MMP-3 (Stromelysin-1). When MMP-7 production is stimulated, native MMP inhibitors (TIMPs) usually follow to quench them. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 10ug/ml. Band at ~30kD Immunohistochemistry (Paraffin-embedded tissue sections): 4-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice