M2420-75
Matrix Metalloproteinase 2 (MMP-2, Collagenase IV, Gelatinase A)
Size
£960.00
- SKU:
- M2420-75
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- N-terminal peptide APSPIIKFPGD-VAPKTDK of procollagenase IV
- Clone:
- 3F260
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M2420-75X
- Extra Details:
- MMPs are proteolytic enzymes capable of degrading connective tissue components. They have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPs and TIMPs play a significant role in regulating angiogenesis. MMP-2; also known as 72kD collagenase IV or gelatinase A is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids into an active form of 62kD. MW of Antigen: 72kD (pro form) Epitope: N-terminal (APSPIIKFPGD-VAPKTDK) Cellular Localization: Cytoplasmic Applications: Suitable for use in Western Blot, Immunohistology and inhibition of MMP-2 actvity. Not suitable for use in Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Inhibition of MMP-2 Activity: 2-4ug/ml (Order Ab without sodium azide). Western Blot: 0.5-1ug/ml for 2hrs at RT Immunohistochemistry: 2-4ug/ml for 30 min at RT (Formalin/paraffin). Note: No special pretreatment is required for the staining of formalin-fixed, paraffin-embedded tissues. Optimal dilutions to be determined by the researcher. Positive Control: Conditioned, serum-free medium from (TPA-treated) HFL-1 cells and placenta. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice