M2420-54

Matrix Metalloproteinase-2 (MMP-2)

Size

£709.00

SKU:: M2420-54
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Activated recombinant human Matrix Metalloproteinase-2
Clone:: 3G186
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: The matrix metalloproteinase (MMP) family is composed of at least 25 zinc-dependent extracellular endopeptidases whose activities are regulated predominantly by expression as inactive precursors, or Zymogens. MMP-2, also known as gelatinase A or 72kD type IV collagenase, plays an important role in processes mediating the degradation of the extracellular matrix (ECM); development, inflammation, tissue repair, tumor invasion, metastasis, etc. Besides a catalytic domain, MMP-2 and the closely related MMP-9 (gelatinase B, 92kD type IV collagenase) contain a hemopexin-like domain and –unique among the metalloproteinases-three in-tandem fibronectin type II (FII) modules which are inserted in their catalytic domain in the vicinity of the active site. Jointly with plasmin, a trypsin-like serine proteinase of narrow substrate specificity, the proteolytic activity of MMP-2 contributes to both the removal of ECM barriers that limit cell movement and in the modulation of cell adhesion, migration, proliferation, and differentiation. Thus, the exposure of multiple independent binding sites is expected to facilitate MMP-2 to “crawl” through the dense extracellular milieu, as the concomitant action of three weakly interactive FII modules should guarantee that MMP-2 preserves the requisite diffusional mobility while remaining in close proximity to its tissue targets. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.1ug/ml Western Blot: 1:500 Immunohistochemistry (Frozen): 5ug/ml Immunohistochemistry (Paraffin): 1:10-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice