M2420-54-ML650
Matrix Metalloproteinase-2 (MMP-2) (MaxLight 650)
Size
£914.00
- SKU:
- M2420-54-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Activated recombinant human MMP-2 protein.
- Clone:
- 3G186
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The matrix metalloproteinase (MMP) family is composed of at least 25 zinc-dependent extracellular endopeptidases whose activities are regulated predominantly by expression as inactive precursors, or Zymogens. MMP-2, also known as gelatinase A or 72kD type IV collagenase, plays an important role in processes mediating the degradation of the extracellular matrix (ECM); development, inflammation, tissue repair, tumor invasion, metastasis, etc. Besides a catalytic domain, MMP-2 and the closely related MMP-9 (gelatinase B, 92kD type IV collagenase) contain a hemopexin-like domain and –unique among the metalloproteinases-three in-tandem fibronectin type II (FII) modules which are inserted in their catalytic domain in the vicinity of the active site. Jointly with plasmin, a trypsin-like serine proteinase of narrow substrate specificity, the proteolytic activity of MMP-2 contributes to both the removal of ECM barriers that limit cell movement and in the modulation of cell adhesion, migration, proliferation, and differentiation. Thus, the exposure of multiple independent binding sites is expected to facilitate MMP-2 to “crawl” through the dense extracellular milieu, as the concomitant action of three weakly interactive FII modules should guarantee that MMP-2 preserves the requisite diffusional mobility while remaining in close proximity to its tissue targets. Applications: Suitable for use in FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: FLISA: 0.1ug/ml Western Blot: 1:500 Immunohistochemistry (Frozen): 5ug/ml Immunohistochemistry (Paraffin): 1:10-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice