M2418-83
Matrilin-4
Size
£787.00
- SKU:
- M2418-83
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gln20-Asp499 (predicted) from human Matrilin-4, expressed in NS0 cells
- Clone:
- 7H224
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Matrilin-4 is a widely expressed 73kD member of the Matrilin family of modular extracellular matrix proteins; it associates with collagen fibrils and is thought to link the fibrils with other matrix constituents. Mouse matrilin-4 contains four EGF domains between two von Willebrand Factor-A domain (vWA) domains. A C-terminal alpha-helix/coiled-coil region is often proteolytically removed so that Matrilin-4 is found as a mixture of monomers with dimers and trimers of Matrilin-1, -2 or -4 molecules. Mature mouse Matrilin-4 shows 90% amino acid identity with human Matrilin-4. A short isoform lacks the N-terminal vWA domain. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml, using recombinant human Matrilin-4, recombinant mouse Matrilin-4 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice