M2415-02C-APC

Mast Cell Tryptase (APC)

Size

£940.00

SKU:: M2415-02C-APC
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human mast cell tryptase purified from human lung tissue.
Clone:: 3H2643 (AA1)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. A substantial quantity of tryptase is estimated to be found in mast cells of skin and lung and suggestes this enzyme plays a major role in mast cell mediated events. In vitro studies indicate tryptase can cleave C3 to form C3a anaphylatoxin, inactivate fibrinogen as a coaguable substrate for thrombin and activate latent collagenase. Human mast cell tryptase has considerable potential as a marker for mast cell activation as well as being a mediator of inflammation. Applications: Suitable for use in FLISA, Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer pH 6.0 is recommended. Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Immunohistochemistry Positive Control Tissue: Tonsil Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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