M2412-50D
Maspin (SERPINB5 , Serpin Peptidase inhibitor, Clade B (ovalbumin), member 5, Peptidase inhibitor 5, PI5, PI-5, Serpin B5)
Size
£812.00
- SKU:
- M2412-50D
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Met1-Pro375 of human Maspin expressed in E. coli. Species Sequence Homology: chimpanzee, 99%; canine, 92%; mouse, 89%; rat, 88%; chicken, 64%
- Clone:
- 9H158
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Serpin B5, also known as Maspin, is a secreted member of the Serpin family although it has no known serine protease inhibitor activity. It is produced by mammary epithelial cells and functions as a tumor suppressor. Serpin B5 is a valuable biomarker of tumor progression and overall prognosis. Applications: Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml. The detection limit for recombinant human Maspin is ~100ng/lane under non-reducing conditions and 25ng/lane under reducing conditions. Immunoprecipitation: 25ug/ml. Conditioned cell culture medium spiked with recombinant human Maspin Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human Maspin is ~15ng/well Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice