M2378-02E-APC

MARCO (Macrophage Receptor MARCO, Macrophage Receptor with Collagenous Structure, AI323439, Ly112, Scara2) (APC)

Size

£1,068.00

SKU:: M2378-02E-APC
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: CHO cells expressing mouse MARCO.
Clone:: 6A441 (ED31)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: MARCO is expressed by distinct populations of macrophages in the spleen and lymph nodes, but is rapidly induced on macrophages in other tissues (e.g. Liver Kupffer cells) during infection or LPS treatment. Clone ED31 binds to the C-terminal cysteine rich domain of MARCO, and has been shown to block ligand binding. Applications: Suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from immunized rats. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.