M2369-51A

MAPRE1 (Microtubule-associated Protein RP/EB Family Member 1, APC-binding Protein EB1, End-binding Protein 1, EB1)

Size

£512.00

SKU:: M2369-51A
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: C-terminal part of mouse EB1 (aa125-268) fused to GST.
Clone:: 10K269
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: MAPRE1 was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:7,000 Immunofluorescence: 1:200-1:1,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.