M2363-22Q

MAP Kinase Kinase 6, Recombinant, Human, GST-Tag (Dual Specificity Mitogen-activated Protein Kinase Kinase 6, MAP2K6, MAPKK 6, MAPKK6, MKK6, PRKMK6, MAPK/ERK Kinase 6, MEK 6, MEK6, Stress-activated Protein Kinase Kinase 3, SAPK Kinase 3, SAPKK3, SAPKK-3, SKK3)

Size

£881.00

SKU:: M2363-22Q
Molecular Weight:: 63.5
Purity:: Purified ≥80%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 3mM DTT, 20% glycerol.
Extra Details:: Source: Recombinant corresponding to aa2-end of human MKK6 with N-terminal GST tag expressed in a Baculovirus infected Sf9 cell expression system (NM_002758.3). Specific Activity: Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, 100ng of inactive MAPK14, 2uM substrate (SER/THR 4) at RT for 1 hour. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.