M2355-10A
MAPKAP Kinase 2 (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2)
Size
£632.00
- SKU:
- M2355-10A
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide in the C-terminal region of human MAPKAPK-2. Species sequence homology: hamster
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- In response to cytokines, stress and chemotactic factors, MAP kinase–activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been illustrated that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four of the residues—Thr25, Thr222, Ser272 and Thr334—are phosphorylated by p38 in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 seems to be essential for the activity of MAPKAPK-2 (2). Thr25 is also phosphorylated by p42 MAPK in vitro and is not required for the activation of MAPKAPK-2 (2). Applications: Suitable for use in Western Blot, Immunoprecipitation and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice