M2354-88N

MAPKAP1 (Mitogen-activated Protein Kinase 2-associated Protein 1, Mitogen-activated Protein Kinase Associated Protein 1, MIP1, Stress-activated Map Kinase-interacting Protein 1, SAPK-interacting Protein 1, mSIN1, SIN1, Target of Rapamycin Complex 2 Subunit MAPKAP1, TORC2 Subunit MAPKAP1)

Size

£655.00

SKU:: M2354-88N
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 18 amino acid peptide from near the center of human MAPKAP1 (GenBank accession no. NP_001006618).
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: MAPKAP1 was initially identified as the human homolog of S. pombe SIN1 (1). Recent evidence has shown that it identical to Mip1, a protein that interacts with MEKK2, a member of the mitogen-activated protein kinase (MAPK) intracellular signaling network. MAPKAP1 is thought to prevent MEKK2 activation and dimerization by forming a complex with the inactive and non-phosphorylated MEKK2, thereby blocking the JNK1/2, ERK1/2, p38 and ERK5 MAPKs (2). MAPKAP1 has also been shown to play a role in the TOR signaling process, a pathway that is involved in controlling cell growth and proliferation in response to environmental cues such as nutrients, growth factors and hormones. Experiments showed that MAPKAP1 helped to maintain the TOR/rictor assembly but not the TOR/RAPTOR complex, which allowed specific phosphorylation of Akt, a kinase that is believed to couple the growth factor-PI3K signaling pathway to the nutrient-regulated TOR signaling pathway (3,4). Multiple alternatively spliced isoforms of MAPKAP1 have been identified. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2 ug/ml. Optimal dilutions to be determined by the researcher. Positive Control: Human skeletal muscle tissue lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice