M2352-01
MAP Kinase p38 alpha (MAPK14, Mitogen-activated Protein Kinase p38 alpha, Mitogen-activated Protein Kinase 14, CSBP, Cytokine Suppressive Anti-inflammatory Drug-binding Protein, CASID-binding Protein, SAPK2A, CSBP, CSBP1, CSBP2, CSPB1, MXI2)
Size
£607.00
- SKU:
- M2352-01
- Purity:
- Purified.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- MBP-p38 MAP kinase recombinant protein.
- Clone:
- 3A31(5F11)
- Format:
- Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- p38 MAP kinase (MAPK) is also called RK (3) or CSBP (2). It is the mammalian homologue of the yeast HOG kinase. It participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP (kinase, alpha, beta, gamma and delta) have been identified. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3 and SEK activate p38 MAP kinase by phosphorylation at threonine 180 and tyrosine 182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8). Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:2000. Incubate membrane with diluted M2352-01 in 1X TBS, 5% nonfat dry milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Flow Cytometry: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice