M2350-01BX

MAP2a,b,c (BSA & Azide Free) (Microtubule Associated Protein 2a,b,c)

Size

£743.00

SKU:: M2350-01BX
Purity:: Purified by Protein G affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Bovine brain MAP2. Cellular Localization: Cytoplasmic. Epitope: aa1-151.
Clone:: 0.N.395
Format:: Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See M2350-01B.
Extra Details:: The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-2ug/ml for 2 hours at RT. Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: IMR-5 or T98G human glioblastoma cells or brain Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.