M2350-01BX-ML650
MAP2a,b,c (BSA & Azide Free) (Microtubule Associated Protein 2a,b,c) (MaxLight 650)
Size
£960.00
- SKU:
- M2350-01BX-ML650
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bovine brain MAP2. Cellular Localization: Cytoplasmic. Epitope: aa1-151.
- Clone:
- 0.N.395
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000 The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 1-2ug/ml for 2hrs at RT. Immunohistochemistry (formalin/paraffin): Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6 for 10-20 min., followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: IMR-5 or T98G human glioblastoma cells or brain. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice