M2256-01
Mannose Receptor (MR, CD206)
Size
£1,138.00
- SKU:
- M2256-01
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human mannose receptor from human placental tissue
- Clone:
- 6A598 (15-2)
- Format:
- Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
- Extra Details:
- The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180kD transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines as IFN-g. Labeling of MR expressing monocytes/macrophages increases at 37°C with prolonged incubation time probably due to internalization of the MR-antibody-complex. The antibody prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific. Applications: Suitable for use in Immunohistochemistry, Western Blot, Flow Cytometry and Functional Studies. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (frozen sections): 1:50, fixed in acetone/chloroform Flow Cytometry: 1:50 Western Blot: 1:50 (non-reduced sample, 6% SDS-PAGE) Functional Studies: M2256-01 functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor (1). Optimal dilutions to be determined by the researcher. Positive Control: Macrophages Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice