M2250-01C

Mannose Binding Protein (MBP, Mannan Binding Lectin, MBL)

Size

£1,138.00

SKU:: M2250-01C
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Serum isolated human RaRF
Clone:: 3E7
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Extra Details:: Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the group of collectins. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASPs that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q–independent. MBL exhibits complement-dependent antibacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of various vertebrate species. Applications: Suitable for use in Western Blot, Immunoassays, Immunohistochemistry, and Flow Cytometry. Can be used for blocking the complement-activating capacity of human MBL. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Western Blot: 1:50 Immunohistochemistry (frozen): 1:50, Tissue sections embedded in ornithine carbamoyltransferase compound were frozen in acetone dry ice and immersed in Tris-buffered saline (TBS) three times for 5 minutes. Treatment in 0.5% hydrogen peroxide in methanol solution has been done to quench endogenous peroxidase activity Optimal dilutions to be determined by the researcher. Negative Control: Nonimmune normal serum or TBS Endotoxin: <24EU/mg Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice