M2196-71B
Munc13-2 (UNC13B, Munc13, Protein Unc-13 Homolog B, UNC13)
Size
£800.00
- SKU:
- M2196-71B
- Application:
- IHC-Fr
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Asp418-Ser1591 of human Munc13-2, expressed in E. coli. Species Sequence Homology: mouse; 95%, rat; 93%
- Clone:
- 10K62
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- UNC13B is a 170kD member of the UNC13 family. It is 1591aa in length and contains three C2 domains, one MHD1 domain, one MHD2 domain, and one phorbol-ester/DAG-type zinc finger. Human UNC13B is 95% and 93% aa identical to mouse and rat UNC13B, respectively. UNC13B is expressed in brain and kidney cortical epithelial and mesangial cells. UNC13B plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. It is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP). In addition, UNC13B may be involved in mediating some of the acute and chronic changes in mesangial cells produced by exposure to hyperglycemia. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml detects recombinant human Munc13-2 in paraffin-embedded human normal and cancer kidney sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice