M2155-17
Maltose Phosphorylase
Size
£914.00
- SKU:
- M2155-17
- Purity:
- Purified by delipidation, salt fractionation and ion exchange chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Maltose Phosphorylase (E. coli)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glycerol. No stabilizing proteins added.
- Extra Details:
- In enzymology, a maltose phosphorylase (EC 2.4.1.8) is an enzyme that catalyzes the chemical reaction maltose + phosphate D-glucose + beta-D-glucose 1-phosphate Thus, the two substrates of this enzyme are maltose and phosphate, whereas its two products are D-glucose and beta-D-glucose 1-phosphate. This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is maltose:phosphate 1-beta-D-glucosyltransferase. This enzyme participates in starch and sucrose metabolism. Applications: Suitable for Immunoblotting (Western or Dot Blot), ELISA, Immunoprecipitation, Conjugation and most immunological methods. Other applications not tested. Recommended Dilution: ELISA: 1:2000-1:10,000; 1ug of Maltose Phosphorylase was used in a standard sandwich ELISA with Rabbit anti-Goat (HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]), A0030, as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher. *Recommended Secondary Antibodies: I1904-28K: IgG, H&L (HRP) Pab RbxGt I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice