M1748-50A
MAG (Myelin associated glycoprotein, GMA, Myelin-associated glycoprotein precursor, Siglec 4A, Siglec 4a, Siglec 4A, S-MAG)
Size
£1,004.00
- SKU:
- M1748-50A
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gly20-Pro516 from rat MAG expresessed in NS0 cells. Species Sequence Homology: mouse; 99%, human; 96%
- Clone:
- 9H156
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Extra Details:
- Myelin-associated glycoprotein (MAG), also known as Siglec-4a, is a 70kD type I transmembrane glycoprotein in the sialoadhesin family. MAG is expressed on myelinating oligodenrocytes and Schwann cells and preferentially binds alpha 2, 3-linked sialic acid on O-linked glycans and gangliosides. MAG functions in the maintenance of oligodendrocytes and myelinated axons. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Capture): 2-8ug/ml. Western Blot: 1ug/ml. The detection limit for rrMAG is ~25ng/lane under non-reucing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice