M1740-55
MafG (v-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog G)
Size
£812.00
- SKU:
- M1740-55
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography from supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Ser162 from human MafG, expressed in E. coli.
- Clone:
- 7H147
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c-Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using HL-60 human acute promyelocytic leukemia cell line and Ramos human Burkitt’s lymphoma cell line. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice