MAD1L1 (Mitotic Arrest Deficient 1-Like 1)

Size

£837.00

SKU:: M1499-08
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: E. coli-derived recombinant human MAD-1L1 (aa1-350) GenBank Accession #NM_003550
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: MAD-1L1 (mitotic arrest deficient 1-like 1) is a member of the spindle-assembly checkpoint (SAC) which prevents anaphase from occurring until all chromosomes are properly aligned at the metaphase plate. Upon checkpoint activation, MAD-1L1 recruits MAD2 to unattached kinetochores, promoting the binding of MAD2 to CDC20, the activator for the anaphase-promoting complex. MAD-1L1 has been implicated to have tumor-suppressing functions, but in small-cell lung cancer cell lines, the MAD-1L1 gene is the most frequent copy number gain, suggesting a more complex role. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.