M1275D
Madcam1 (Mucosal Addressin Cell Adhesion Molecule 1, MAdCAM-1, mMAdCAM-1)
Size
£473.00
- SKU:
- M1275D
- Application:
- Flow Cytometry
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Endothelial cells
- Clone:
- MECA-367
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- MAdCAM-1 is a 58-66kD type I glycoprotein, also known as Mucosal addressin cell adhesion molecule-1. This mucosal vascular addressin is a member of the Ig superfamily found on fetus and neonatal endothelial cells. In adults, MAdCAM-1 is predominately expressed on high endothelial venule (HEV) of Peyer's patches, mesenteric lymph nodes and gut lamina propria. It is also expressed on vascular endothelium in mammary glands and pancreas. MAdCAM-1, through its interaction with integrin a4b7 or CD62L, is involved in lymphocyte tethering, rolling, and homing. It has been reported that immobilized MAdCAM-1 is able to co-stimulate T cell proliferation. This antibody blocks the interaction of MAdCAM-1 with its counter-receptor both in vitro and in vivo. In vivo administration of this monoclonal antibody is able to reduce T-cell mediated inflammation in some gastrointestinal diseases. Applications: Suitable for use in Flow Cytometry. Though not tested, Immunohistochemistry (frozen), Immunoprecipitation and Western Blot have been reported in literature (ref 1,5 and 6). Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.25ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice