M1250-01C
Macrophages
Size
£576.00
- SKU:
- M1250-01C
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Porcine alveolar macrophages
- Clone:
- BA4D5
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- Macrophages and histiocytes, which are widely distributed throughout the body, play a crucial role in the body defense mechanisms. They are heterogenous in morphology, cytochemistry, function and surface antigens. Immunohistochemical labeling with monoclonal antibodies is one of the most specific and reliable techniques for the identification and localization of these cells. Monoclonal antibodies to macrophage and histiocyte surface or cytoplasmic antigens are important for the immunodiagnosis of both benign and malignant histiocytic lesions. They are useful in the investigation of macrophage differentiation and maturation and in the diagnosis of various inflammatory, reactive and neoplastic disorders of macrophages. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required. Immunohistochemistry: Frozen sections Western Blot: Non-reducing conditions. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Recommended Negative Control: I1904-84Q3: IgG2b Murine Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice