M1202-76
Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen)
Size
£563.00
- SKU:
- M1202-76
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human monoocytes. UniProt Accession #: P06702
- Clone:
- 3H2617 (MAC387)
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Extra Details:
- The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100; 10ul lables 1x10e6 cells in 100ul. Membrane permeabilization is required for this application. Immunohistochemistry (Frozen and Paraffin): 1:100-1:200; Prior to staining paraffin sections, product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment. Frozen sections do not require pretreatment. Optimal working dilutions to be determined by researcher. Recommended Positive Control: Human spleen Recommended Negative Control: I1904-78R: IgG1: Murine Negative Control Recommended Secondary Antibodies: I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo I1904-66C: IgG, F(ab’)2 X-adsorbed (HRP) Pab Rb x Mo I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb x Mo Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice