M1202-76-ML650
Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen) (MaxLight 650)
Size
£837.00
- SKU:
- M1202-76-ML650
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human monocytes
- Clone:
- 3H2617 (MAC387)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Membrane permeabilization is required for this application. Immunohistochemistry (Frozen and Paraffin): Prior to staining paraffin sections, product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment. Frozen sections do not require pretreatment. Optimal working dilutions to be determined by researcher. Recommended Positive Control: Human spleen Recommended Negative Control: I1904-78R: IgG1: Murine Negative Control Recommended Secondary Antibodies: I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo I1904-66C: IgG, F(ab’)2 X-adsorbed (HRP) Pab Rb x Mo I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb x Mo Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice