M1202-66
Macrophage Inflammatory Protein 3 beta, Human (MIP-3b, Exodus-3, ELC)
Size
£479.00
- SKU:
- M1202-66
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human MIP-3 beta, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.2.
- Extra Details:
- MIP-3 beta acts as a chemoattractant for T and B lymphocytes, dendritic cells, NK cells and myeloid progenitor cells, through specific binding to CCR7, a G-protein coupled receptor known to be up-regulated by Epstein-Barr virus infected B cells and Herpes virus infected T cells. A correlation between the response to MIP-3 beta and the level of CCR7 expression and has been revealed in the maturation of dendritic cells (DCs), and increased levels of both MIP-3 beta and CCR7 expression have been shown in bronchoalveolar lavage fluid (BALF) and BALF cells from patients with the granulomatous disorder sarcoidosis, associated predominantly with CD4+ T lymphocyte alveolitis, and hence T cell recruitment. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA (sandwich): 0.5-2ug/ml. (100ul/well), in conjunction with M1202-65K as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human MIP-3β. Western Blot: 0.1-0.2ug/ml. The detection limit is 1.5-3ng/lane under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice