M1202-50C

Macrophage Inflammatory Protein 2, Recombinant, Mouse (MIP2, MIP-2, Chemokine (C-X-C Motif) Ligand 2, C-X-C Motif Chemokine 2, CXCL2, CINC-2a, GRO2, GROb, MIP-2a, MGSA-b, Scyb, SCYB2)

Size

£544.00

SKU:: M1202-50C
Molecular Weight:: 7.8
Purity:: ≥98% (SDS-PAGE, HPLC). Endotoxin: ≤0.1ng/ug (<1EU/ug)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 10mM Acetic Acid. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Add 0.1% HSA or BSA for long term storage. Do not vortex.
Extra Details:: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant mouse MIP-2 is a 7.8kD protein consisting of 73 amino acids, including the 'ELR' motif common to the CXC chemokine family that binds to CXCR1 or CXCR2. Recombinant protein corresponding to mouse MIP-2, expressed in E. coli Molecular Weight: ~7.8kD Amino Acid Sequence: AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN Biological Activity: Determined by its ability to chemoattract total human neutrophils using a concentration range of 1-10ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.