M1201-53
Macrophage Derived Chemokine (MDC, CCL22)
Size
£466.00
- SKU:
- M1201-53
- Purity:
- Purified by Protein G affinity chromatography from cell culture
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Macrophage Derived Chemokine expressed in E. coli
- Clone:
- 4i23 (1.3_1C6-1D3)
- Format:
- Supplied as a lyophilized powder from PBS. No preservatives added. Reconstitute with 500ul sterile ddH2O.
- Extra Details:
- Human Macrophage-Derived Chemokine (MDC) is a unique member of the CC chemokine family. Human MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells or several lines of epithelial, endothelial or fibroblast origin. Recombinant Human MDC is an 8kD protein containing 67aa residues. Applications: Suitable for use in ELISA. Other applications have not been tested. Recommended Dilutions: ELISA (Sandwich): 2-4ug/ml (100ul/well) detects at least 100pg/ml of recombinant human Macrophage Derived Chemokine when used with M1201-54, Macrophage Derived Chemokine (MDC) (Biotin) as detection antibody at a concentration of 1-2ug/ml. Optimal dilutions to be determined by the researcher. Hybridoma: ICR F1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice