L9200-08
Lysozyme (Muramidase)
Size
£704.00
- SKU:
- L9200-08
- Purity:
- Purified by ion exchange chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified hen egg lysozyme.
- Clone:
- 5C74
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O.
- Extra Details:
- Lysozyme is a relatively small (129aa) secretory enzyme that catalyzes the hydrolysis of b-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers. Lysozyme is common in animals and plants. In birds, lysozyme is also an exceptionally abundant protein in egg whites. Its biological function in fowl eggs is unclear. Hen egg white lysozyme "c" is the most commonly studied form and source of the enzyme. Lysozyme from domestic goose is designated lysozyme "g". Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leukocytes, kidneys and urine, where it probably serves as an anti-bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacterial susceptible to osmotic lysis. Plant lysozyme is found in ficus and papaya latex, and chemically is distinct from the egg white enzyme. Applications: Suitable for use in Direct ELISA. Other applications not tested. Recommended Dilutions: Direct ELISA: 1:5000 dilution will yield 0.2 O.D. IgG (AP) rabbit anti-mouse. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice