L9190-20
Lysosome Associated Membrane Protein 3 (LAMP 3, Dendritic Cell Lysosome Associated Membrane Protein, DC-LAMP, CD208)
Size
£850.00
- SKU:
- L9190-20
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Asp21-Thr381 of human DC-LAMP lumenal domain expressed in NS0 cells. Species sequence homology: canine 72%, mouse 55%.
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- DC-LAMP (dendritic cell lysosome-associated membrane protein: also known as CD208 and LAMP3) is a 50-70kD member of the LAMP family of proteins. Mature human DC-LAMP is a 389 amino acid type I transmembrane protein. It has a 254aa lumenal N-terminus and a short 14aa cytoplasmic tail. The molecule is found in type II pneumocytes, interdigitating DC, and various tumors. DC-LAMP serves as a marker of human mature DC, and it may play a role in normal lysosome and endosome function. Over the region used as immunogen, human DC-LAMP shares 72% and 55% aa sequence identity with canine and mouse DC-LAMP, respectively. Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications have not been tested. Recommended Dilution: Western Blot: 1ug/ml Flow Cytometry: 0.25ug/10e6 cells. For mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+cells Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice