L9190-10J
LAMP1 (Lysosomal-associated Membrane Protein 1 CD107a, CD107a Antigen, LAMP-1, LAMPA, LGP120, Lysosome-associated Membrane Glycoprotein 1 Precursor)
Size
£882.00
- SKU:
- L9190-10J
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ala28-Asn380 from human LAMP1 expressed in NSO cells.
- Clone:
- 11D69
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Lysosome-associated membrane protein-1(LAMP1), also known as CD107a, is a 100-130kD member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389aa type I transmembrane glycoprotein. It contains a 354aa luminal/extracellular domain (ECD) (aa28-381) and a 12aa cytoplasmic tail (aa405-416). The ECD has two large looping regions (aa28-193 and 227-381) plus multiple N-and O-linked glycosylation sites. There is one potential splice variant that shows a 26aa substitution in the signal sequence. Over (aa28-380), human LAMP1 shares 64% aa identity with mouse LAMP 1. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry (Intracellular Staining): 2.5ug/ml labels 10e6 cells using THP‑1 human acute monocytic leukemia cell line fixed with paraformaldehyde and permeabilized with saponin Western Blot: 2ug/ml Immunocytochemistry: 8-25ug/ml using immersion fixed THP-1 human acute monocytic leukemia cell line Immunohistochemistry: 8-25ug/ml with immersion fixed paraffin-embedded sections of human kidney. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice