L9058-02D

Lysine, Acetylated (Acetyl-Lysine)

Size

£320.00

SKU:: L9058-02D
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Acetylated protein mixture
Clone:: 15G10
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Proteins are reversibly and dynamically acetylated on the e-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetytransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. This antibody recognizes acetylated lysine residues on proteins. Applications: Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 1-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.